Quantification of tryptophan metabolites in the kynurenine pathway

The kynurenine pathway is the major metabolic route for tryptophan degradation.

This pathway generates several important metabolites involved in inflammation, immune response, and excitatory neurotransmission.

Our targeted analysis provides sensitive and accurate quantification of 10 metabolites in plasma, serum and urine samples using UPLC-triple quadrupole MS.

Sample Requirements

Sample type	Requirements
Plasma/Urine	50 µL/25 µL

Target analyte sensitivity

Target analyte	Plasma/Serum	Urine
Anthranilic acid	0.016 uM	0.016 uM
Kynurenic acid	0.016 uM	0.016 uM
Kynurenine	0.016 uM	0.016 uM
Picolinic acid	0.006 uM	0.006 uM
Quinolinic acid	0.063 uM	0.063 uM
Serotonin	0.008 uM	0.016 uM
Tryptophan	0.156 uM	0.156 uM
Xanthurenic acid	0.016 uM	0.016 uM
3-OH-anthranilic acid	0.016 uM	0.016 uM
3-OH-kynurenine	0.016 uM	0.016 uM

Instrumentation

Waters UPLC I-Class
Waters XEVO TQ-XS

Method reference

https://www.sciencedirect.com/science/article/pii/S2589004221013249?via%3Dihub