Quantification of tryptophan metabolites in the kynurenine pathway
The kynurenine pathway is the major metabolic route for tryptophan degradation.
This pathway generates several important metabolites involved in inflammation, immune response, and excitatory neurotransmission.
Our targeted analysis provides sensitive and accurate quantification of 10 metabolites in plasma, serum and urine samples using UPLC-triple quadrupole MS.
Sample Requirements
| Sample type | Requirements |
|---|---|
| Plasma/Urine | 50 µL/25 µL |
Target analyte sensitivity
| Target analyte | Plasma/Serum | Urine |
|---|---|---|
| Anthranilic acid | 0.016 uM | 0.016 uM |
| Kynurenic acid | 0.016 uM | 0.016 uM |
| Kynurenine | 0.016 uM | 0.016 uM |
| Picolinic acid | 0.006 uM | 0.006 uM |
| Quinolinic acid | 0.063 uM | 0.063 uM |
| Serotonin | 0.008 uM | 0.016 uM |
| Tryptophan | 0.156 uM | 0.156 uM |
| Xanthurenic acid | 0.016 uM | 0.016 uM |
| 3-OH-anthranilic acid | 0.016 uM | 0.016 uM |
| 3-OH-kynurenine | 0.016 uM | 0.016 uM |
Instrumentation
- Waters UPLC I-Class
- Waters XEVO TQ-XS